X.B: Esterase Stains

Graphic Jump Location

View Full Size||Download Slide (.ppt)

X.B.001

Acute monocytic leukemia. Double esterase stain. Marrow film. Leukemic monocytic cells, the dominant cell population, stain with gray-black color. Two admixed immature neutrophils stain with reddish-brown color. Double esterase combines two substrates: naphthol AS-D chloracetate that reacts with primary granules in the neutrophilic series and α-naphthyl butyrate that stains material in the endoplasmic reticulum of cells in the monocytic lineage.

Graphic Jump Location

View Full Size||Download Slide (.ppt)

X.B.002

Acute myelomonocytic leukemia. Double esterase stain. Marrow film. Leukemic monocytes stain gray-black (e.g. arrow) and the leukemic granulocytes stain reddish-brown (e.g. asterisk). Double esterase combines two substrates: naphthol AS-D chloracetate that reacts with primary granules in the neutrophilic series and α-naphthyl butyrate that stains material in the endoplasmic reticulum of monocytic lineage cells.

Graphic Jump Location

View Full Size||Download Slide (.ppt)

X.B.003

Acute Myelomonocytic Leukemia. Marrow Film. Leukemic monocytes stain gray-black and leukemic granulocytes stain reddish-brown. Double esterase combines two substrates: naphthol AS-D chloracetate that reacts with primary granules in the neutrophilic series and a-naphthyl butyrate that stains structures in the endoplasmic reticulum of monocytic cells. (A,B) Each marrow area has two monocytic lineage cells and three granulocytic (neutrophilic) lineage cells.