X.C: Immunostains

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X.C.001

CD3 immunostain of T lymphocytes. This reddish-brown reaction product identifies T lymphocytes. This image shows staining of a background of polyclonal reactive T lymphocytes amidst the centroblasts of large B cell lymphoma (e.g., asterisks).

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X.C.002

CD10 immunostain of germinal center B lymphocytes. This reddish-brown reaction product identifies, in this case, neoplastic follicular B cells, an immunophenotype that resembles normal germinal center cells.

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X.C.003

CD15 immunostain of Reed-Sternberg cells. The reddish-brown reaction product stains the cytoplasm of Reed-Sternberg cell (large binucleate cell in center of field) (arrow) and Reed-Sternberg variant cell (smaller mononuclear cells with reddish-brown reaction product in the cytoplasm) (asterisk) in a case of classical Hodgkin lymphoma.

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X.C.004

CD20 immunostain of B lymphocytes. The reddish-brown reaction product stains neoplastic lymphocytes with a B cell phenotype. Reactive T lymphocytes are unstained.

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X.C.005

CD30 immunostain of Reed-Sternberg cells. This reddish-brown reaction product identifies Reed-Sternberg and Reed-Sternberg variant cells in Hodgkin lymphoma. Note classical binucleate Reed-Sternberg cell (e.g., arrow) and mononuclear variant Reed-Sternberg cells (e.g., asterisk). This immunostain also reacts with anaplastic large cell lymphoma and activated lymphocytes.

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X.C.006

CD34 stain for myeloblasts. Marrow biopsy. (A) Marrow shows replacement by monotonous population of blast cells. (B) Anti-CD34 staining of blast cells confirms the myeloblastic nature of the acute leukemia.

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X.C.007

CD45R0 immunostain of T lymphocytes. This reddish-brown reaction product identifies the T cell origin of the tumor cells, as in this case of angioimmunoblastic T-cell lymphoma.

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X.C.008

A patient with myeloma undergoing chemotherapy. (A).Marrow biopsy stained with H & E. Occasional plasma cells noted.(B) Immunostain for CD138 (syndecan-1) shows numerous plasma cells. (C)Immunostain for lambda light chain indicates virtually all plasma cells expressing lambda light chains (D)Immunostain for kappa light chains. Only a rare positive plasma cells confirming substantial monoclonal plasma cell infiltrate (monoclonal lambda light chain type).