TY - CHAP M1 - Book, Section TI - Lymphocytic Leukemia and Lymphoma A1 - MA, Lichtman A1 - MS, Shafer A1 - RE, Felgar A1 - N, Wang PY - 2017 T2 - Lichtman's Atlas of Hematology 2016 AB - Graphic Jump LocationView Full Size||Download Slide (.ppt)IX.C.001Precursor T-cell acute lymphoblastic leukemia/lymphoma. Flow cytometry. Staining pattern seen in a marrow cells from a patient with acute lymphoblastic leukemia/lymphoma, precursor T-cell type. The upper left panel shows the pattern of leukocyte common antigen (CD45) versus side-scatter analysis. Lymphoblasts have slightly dimmer CD45 staining than mature lymphocytes but merges with the normal lymphocyte population. The upper middle and upper right panels show that the bright CD45+ population (indicated by R1 and shown in red on gated graphs) contains a subpopulation of cells that are negative for surface CD3 and surface CD19 but co-express CD4 and CD8 (a common thymocyte phenotype). In this case, the CD4+/CD8+ population accounts for approximately 26% of all cells in the marrow. The lower row of dot plots indicates that this population is surface CD3 negative, surface CD5 positive, surface CD7 positive, and co-expresses cytoplasmic CD3 and terminal deoxynucleotidyl transferase (TdT). TdT is a nuclear antigen, but can be assessed by flow cytometry using permeabilization methods that are also used for assessing cytoplasmic marker staining (cellular fixation in formalin, followed by incubation of antibody with cells in a very weak detergent solution). SN - PB - McGraw-Hill Education CY - New York, NY Y2 - 2024/03/28 UR - hemonc.mhmedical.com/content.aspx?aid=1138038834 ER -