RT Book, Section A1 MA, Lichtman A1 MS, Shafer A1 RE, Felgar A1 N, Wang SR Print(0) ID 1138037650 T1 Myeloid Precursors T2 Lichtman's Atlas of Hematology 2016 YR 2017 FD 2017 PB McGraw-Hill Education PP New York, NY SN 9780071602679 LK hemonc.mhmedical.com/content.aspx?aid=1138037650 RD 2024/03/29 AB Graphic Jump LocationView Full Size||Download Slide (.ppt)V.D.001Band and segmented neutrophil. Marrow film. The band neutrophil has the same cytoplasmic appearance as its predecessor, the neutrophilic metamyelocyte, that is a tan coloration throughout the cytoplasm, reflecting the neutral staining properties of the neutrophilic granules, not individually discernible because they are less than 0.5 μm in diameter. The sole distinguishing feature of this state of maturation is the sausage shaped nucleus with a thickness that is nearly identical from one end of the nucleus to the other with no hint of segmentation. A segmented neutrophil sits adjacent to the band neutrophil. Note the clear segmentation with one lobe being connected to the remainder of the nucleus by a very thin strand. Note irregularities in the main body of the nucleus with minor and major constrictions and another developing segment apparent at the right end of the nucleus. The nuclear chromatin is more condensed and darker than that of the band neutrophil. A small lymphocyte is at the bottom of the field. In supravital preparations nuclear segmentation is a dynamic event with changing lobe numbers and areas of segmentation.